Protein purification methods

Your guide to protein purification basics, tips & tricks, and more

Protein sample preparation

Tips for using protein sample prep techniques such as precipitation, filtration, desalting, buffer exchange, and centrifugation.

Antibody purification

Antibody purification

Capture polyclonal and monoclonal antibodies (mAb), and antibody fragments, using affinity resins.

His_tagged proteins

His-tagged proteins

Read about the advantages of histidine tagged protein purification and how it works.

GST_tagged proteins

GST-tagged proteins

Learn when to use glutathione S-transferase (GST) tags and how to purify GST-tagged proteins.

Hydrophobic interaction chromatography

Hydrophobic interaction chromatography

Learn about hydrophobic interaction chromatography (HIC), reversed phase chromatography (RPC), and the difference between the techniques.

Protein purification FAQs

Questions about protein purification covering sample prep, tagged protein purification, and different purification techniques.

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