Protein purification videos

Watch our videos and tutorials to get protein purification tips and advice

Protein purification principles

Affinity chromatography – principles

Affinity chromatography is a powerful technique that separates proteins based on the reversible interaction between the target protein and binding substance (ligand).

Hydrophobic interaction chromatography principles

Hydrophobic interaction chromatography separates biomolecules according to differences in their hydrophobicity. Conditions are relatively mild.

Removing affinity tags from tagged proteins

An affinity tag can be removed while a tagged recombinant protein is bound to affinity media (resins) or after elution. This video provides examples of automated on-column tag removal with different proteases.

Ion exchange chromatography – principles

Ion exchange chromatography (IEX), which includes cation exchange chromatography (CIEX) and anion exchange chromatography (AIEX), separates molecules according to differences in net surface charge.

Size exclusion chromatography – principles

Size exclusion chromatography, or gel filtration, is the simplest and mildest liquid chromatography technique. This technique separates molecules based on differences in size.

Rapid size exclusion chromatography

Quickly analyze purified antibodies or other proteins using our prepacked Superdex and Superdex Increase columns. This video provides examples of purity checking and other applications for our size exclusion chromatography columns.

Protein purification systems for research

ÄKTA pure - Protein purification your way

Flexible and intuitive chromatography system to meet your purification challenges in research applications.

Using ÄKTA pure – a scientist’s perspective

Lou Fabri’s protein production group at CSL processes approximately 1500 proteins each year. In this video Dr Fabri summarizes his conclusions from using ÄKTA pure to automate a two-step protein purification process.

ÄKTA start – how to perform a purification run

See how easy it is to setup and perform a protein purification run using the standalone system.

ÄKTA avant – overview

ÄKTA avant is a preparative chromatography system designed for fast and secure development of scalable methods and processes. With UNICORN software, ÄKTA avant offers a complete solution for productivity in process development.

ÄKTA start protein purification system – overview

ÄKTA start is a compact, easy-to-use system that provides an affordable alternative to manual protein purification. The easy-to-use design, with a front-facing working area, allows easy access.

ÄKTA avant – system features

ÄKTA avant is a preparative chromatography system designed for fast and secure development of scalable methods and processes. This video highlights the many features that ÄKTA avant offers.

Protein purification how-to videos

HiTrap Protein A & Protein G column - Tips & Tricks

Isolate IgG and IgG subclasses from cell culture supernatants and serum with HiTrap Protein G HP and HiTrap Protein A HP columns. Maximize your results with the tips and tricks in this video.

HisTrap FF crude columns - Tips & Tricks

HisTrap FF crude columns allow you to purify histidine-tagged (his-tagged) proteins directly from unclarified lysates. Maximize your results with the tips and tricks in this video.

Rapid gel filtration - Tips & Tricks

Analyze samples of purified antibody in 15 minute runs using GE Healthcare Life Sciences prepacked size exclusion chromatography columns.

How to pack a HiScale column

These pressure-stable columns are designed for high flow agarose media (resins) such as MabSelect and Capto. HiScale columns are available in a number of sizes and can handle chromatography media up to 785 ml.

Attach a HiTrap column to a chromatography system

Prepacked HiTrap columns are designed for fast and easy small-scale preparative protein purifications. All you need is a HiTrap column and a syringe, pump, or chromatography system. Columns can be connected in series for simple scale-up.

UNICORN 7 tutorial – peak area calculations

Learn how to adjust the baseline range and peak limits using UNICORN software evaluation module. Also, learn how to use the extinction coefficient to calculate the amount and concentration of protein in peaks.

ÄKTA system tutorial: Avoid getting air into the column

Step-by-step instructions on connecting a size exclusion chromatography column drop-to-drop to avoid getting air into your column.

ÄKTA system tutorial: Determine column efficiency

Run and evaluate column performance test to determine efficiency of your packed column.

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