GE offers four categories of size exclusion chromatography (SEC) media with resolution in the following order: Superdex > Superose >Sephacryl > Sephadex. A wide range of product options are available, including prepacked columns for chromatography systems, prepacked formats for manual purification, and loose media (resins).
Sephadex is used for group separations, such as desalting and buffer exchange. Sephadex LH-20 medium can be used with organic solvents and is used for example in Traditional Chinese Medicine (TCM). The other media are used for high-resolution fractionation to check protein purity or to further purify a partially purified or almost pure protein.
Superose prep grade media have a very wide fractionation range while Sephacryl and Superdex prep grade media is well-suited for fast, high recovery separations at laboratory and industrial scale, with Superdex giving the highest resolution.
Superdex Increase and Superose Increase are the next new generation of agarose-based SEC media for small-scale preparative purification and analysis (sample volumes up to 500 µl), offering improved performance in terms of higher resolution and shorter runtimes due to smaller and more rigid beads. They will eventually replace their Superdex and Superose predecessors.