New generation SEC columns

GE Healthcare expands the range with the addition of Superdex 30 Increase

Our new generation size exclusion chromatography columns called “Increase” offer improved performance than their predecessors.

Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity, they offer:

  • Reduced runtimes with maintained resolution for results faster
  • Higher resolution for improved purity
  • Higher lot to lot consistency

With the addition of Superdex 30 Increase, you now have four choices of the new generation SEC resin for small-scale preparative purification and analysis.

Discover Superdex 30 Increase now

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Woman working with Superdex 75 Increase column

What our customers are saying

Superose 6 Increase 10/300 GL column

Dr. Tomohiro Nishizawa
@ Nureki Laboratory, Department of Biological Sciences, Graduate School of Science, The University of Tokyo

"Now I can work on more samples, resulting in higher throughput. I can now purify a protein in 30 minutes instead of an hour

We asked Dr. Tomohiro Nishizawa, who frequently purifies membrane proteins for crystallization, how he likes the new size exclusion chromotography column Superose 6 Increase. He is pleased with the shorter purification time and higher throughput compared with standard Superose 6 columns.

Superdex 200 Increase, Superose 6 Increase

Ciaran McKeown
@Department of Medicine, Imperial College London

"We use SEC MALS (Size Exclusion Chromatography Multi Angle Light Scattering) to determine absolute molecular weight for proteins and protein complexes. It is a very powerful technique and for the SEC part we need a column that will give us good separation and good resolution. We have tried a column that we bought with the system, this gives reasonable results but fractionation range was limiting.

Superdex 200 Increase 3.2/300 column

Meindert H. Lamers - Medical Research Council
@ Laboratory of Molecular Biology, Cambridge Medical Campus

"We have tested the new Superdex 200 Increase (3.2/300) column and were very impressed with its performance. The resolution is much improved when compared to the standard Superdex 200 PC (3.2/30) column.

Notably, we can see defined peaks in mixtures of proteins, when before these would merge into one single peak.

Superdex 200 Increase

Andreas Lehmann
@ Molecular Partners AG

"We use the Superdex 200 increase 5/150 for screening purposes. Since the switch to this newer generation of Superdex we have analyzed more than 10’000 samples. I really like the speed and consistent behavior which helped us to increase the throughput of this process step."

Learn more about Superdex 200 Increase

Superdex 75 Increase 10/300 GL

Ian A Taylor
@ Macromolecular Structure Laboratory, Francis Crick Institute, London

"Even at twice the flow rate, resolution is still better than with the regular Superdex 75 column. Only minimal increases in peak broadening are observed with increasing flow rate (0.5, 0.8 and 1.0 ml/min). In conclusion, the new 10/300 column offers significant improvements over the Superdex 75 (10/300) column. It means we can conduct MALLS runs in half the time and with increased chromatographic resolution. The increased backpressure is not a significant problem if using a chromatography system that manages these pressures adequately."

Learn more about Superdex 75 Increase

Superdex 75 Increase 10/300 GL

Dr Paul Wan
@ Discovery Sciences, AstraZeneca R&D, UK

"The resolution was much better than with the standard Superdex 75 resin. Increasing the flow rate (0.5, 0.75, 1.5 ml/min) didn’t seem to affect the good resolution on the column. I was amazed at how little loss in resolution I got when increasing the flow rate (0.5, 0.75, 1.5 ml/min). The runs were so much faster than before"

Learn more about Superdex 75 Increase

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