5 beginner tips for getting his-tagged protein that meets your needs
November 15, 2015
It seems like everyone is purifying histidine-tagged (his-tagged) proteins, so how hard can it be? Affinity purification of these proteins is not difficult, but it is not foolproof. There are some simple steps that you can take before, during, and after purification to ensure that you get protein that will work for your application.